Bio-enhanced removal of hydrocarbon contents from spent engine oil contaminated soil using Staphylococcus aureus and Bacillus cereus co-culture


 The study assessed the removal of total petroleum hydrocarbon (TPH) and polyaromatic hydrocarbons (PAHs) from spent engine oil (SEO) contaminated soil through bioenhancement bacteria isolated SEO polluted soil. Sterilized was subjected to a three level contamination before addition sterilized biostimulants including powdered cow dung (CD), cocoa pod husk (CPH) compost (made fresh CPH CD). Bacterial inoculum being Staphylococcus aureus Bacillus cereus co-culture (150 mL) added mixture in polyethylene bags. It factorial experiment that laid out completely randomized design (CRD). TPH PAHs were estimated first day, fifth week tenth room incubation lasted. Results generated influence on degradation potential bacterial showed contents significantly enhanced (p < 0.05). At week, most reductions (315 380 mg kg–1) compared with other 5% 15% levels, respectively. Compost equally (48.8, 39.6 94.6 5%, 10% levels However, quantity degraded higher bioaugmented biostimulated samples control employed. technology adopted this can be effectively employed for bioremediation related pollution.